Her facial features consist of a small golden nose, round eyes with three eyelashes on each and a yellow mole under her right eye. Chalice is an anthropomorphic light yellow chalice, with a golden lip and handles, and a golden-and-white-striped straw sticking out of her head, similar to that of Mugman. She is supposedly on a 'secret mission' that involves helping Chef Saltbaker bake the Wondertart. In her corporeal form, however, she serves as a tritagonist ready to aid Cuphead and Mugman in their adventure.
In her Legendary Chalice form, her role in the game is basically that of a 'damsel in distress' whom the player has to rescue in order to obtain Super Arts. In the main game, she is known as The Legendary Chalice - an NPC encountered in the Mausoleum levels.